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resource s ion exchange column  (Danaher Inc)


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    Structured Review

    Danaher Inc resource s ion exchange column
    Resource S Ion Exchange Column, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 993 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/resource+s+ion+exchange+column/RESOURCE+S/pmc09110413-205-14-19
    Average 96 stars, based on 993 article reviews
    resource s ion exchange column - by Bioz Stars, 2026-10
    96/100 stars

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    Chromatography:

    Article Title: The Differential Affinity of the Usher for Chaperone-Subunit Complexes is Required for Assembly of Complete Pili
    Article Snippet: .. The samples were then subjected to an additional chromatography step using a Resource S ion exchange column (GE Healthcare) and eluted using a NaCl gradient. ..

    Affinity Column:

    Article Title: Pharmacokinetics of Naja sumatrana (Equatorial Spitting Cobra) Venom and Its Major Toxins in Experimentally Envenomed Rabbits
    Article Snippet: .. Resource S ion exchange column and HiTrap Protein A HP affinity column were purchased from GE Healthcare (New Jersey, USA). .. Goat anti-rabbit IgG-horseradish peroxidase (HRP) conjugate was obtained from Abnova, Taipei, Taiwan.

    Concentration Assay:

    Article Title: Insights from biochemical reconstitution into the architecture of human kinetochores.
    Article Snippet: .. CENP-HI57-CKM in solution was then adjusted to a salt concentration of 100 mM and a pH of 6.5, before loading on a 6 ml Resource S ion-exchange column (GE Healthcare), equilibrated in 20 mM MES 6.5, 100 mM NaCl, 2 mM β-mercaptoethanol. .. CENP-HI57-CKM was then eluted with a gradient of 100–1,000 mM NaCl over 20 column volumes, and peak fractions corresponding to CENP-HI57-CKM were pooled and concentrated in a 50 kDa MW Amicon concentrator (Millipore).

    Filtration:

    Article Title: CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex
    Article Snippet: Cleared lysates of His-CTNNBL1, GST-CWC15 and MBP-CDC5L were incubated with Ni-NTA (Qiagen), Glutathione-Sepharose 4B (GE Life Sciences) or Amylose resin (New England Biolabs) respectively, for 1 h at 4°C, washed and eluted using 1M imidazole, 10 mM reduced glutathione or 50 mM maltose, respectively. .. For gel filtration, crosslinking and HDX, the tags of CWC15 and CDC5L were cleaved off by HRV 3C and Tobacco Etch Virus (TEV) proteases respectively, followed by a second purification step on a 6 ml Resource Q ion exchange column in the case of CTNNBL1 and CWC15, or a Resource S ion exchange column in the case of CDC5L using an AKTA Prime Plus FPLC system (GE Lifesciences). ..

    Structural Proteomics:

    Article Title: CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex
    Article Snippet: Cleared lysates of His-CTNNBL1, GST-CWC15 and MBP-CDC5L were incubated with Ni-NTA (Qiagen), Glutathione-Sepharose 4B (GE Life Sciences) or Amylose resin (New England Biolabs) respectively, for 1 h at 4°C, washed and eluted using 1M imidazole, 10 mM reduced glutathione or 50 mM maltose, respectively. .. For gel filtration, crosslinking and HDX, the tags of CWC15 and CDC5L were cleaved off by HRV 3C and Tobacco Etch Virus (TEV) proteases respectively, followed by a second purification step on a 6 ml Resource Q ion exchange column in the case of CTNNBL1 and CWC15, or a Resource S ion exchange column in the case of CDC5L using an AKTA Prime Plus FPLC system (GE Lifesciences). ..

    Virus:

    Article Title: CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex
    Article Snippet: Cleared lysates of His-CTNNBL1, GST-CWC15 and MBP-CDC5L were incubated with Ni-NTA (Qiagen), Glutathione-Sepharose 4B (GE Life Sciences) or Amylose resin (New England Biolabs) respectively, for 1 h at 4°C, washed and eluted using 1M imidazole, 10 mM reduced glutathione or 50 mM maltose, respectively. .. For gel filtration, crosslinking and HDX, the tags of CWC15 and CDC5L were cleaved off by HRV 3C and Tobacco Etch Virus (TEV) proteases respectively, followed by a second purification step on a 6 ml Resource Q ion exchange column in the case of CTNNBL1 and CWC15, or a Resource S ion exchange column in the case of CDC5L using an AKTA Prime Plus FPLC system (GE Lifesciences). ..

    Purification:

    Article Title: CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex
    Article Snippet: Cleared lysates of His-CTNNBL1, GST-CWC15 and MBP-CDC5L were incubated with Ni-NTA (Qiagen), Glutathione-Sepharose 4B (GE Life Sciences) or Amylose resin (New England Biolabs) respectively, for 1 h at 4°C, washed and eluted using 1M imidazole, 10 mM reduced glutathione or 50 mM maltose, respectively. .. For gel filtration, crosslinking and HDX, the tags of CWC15 and CDC5L were cleaved off by HRV 3C and Tobacco Etch Virus (TEV) proteases respectively, followed by a second purification step on a 6 ml Resource Q ion exchange column in the case of CTNNBL1 and CWC15, or a Resource S ion exchange column in the case of CDC5L using an AKTA Prime Plus FPLC system (GE Lifesciences). ..

    Fast Protein Liquid Chromatography:

    Article Title: CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex
    Article Snippet: Cleared lysates of His-CTNNBL1, GST-CWC15 and MBP-CDC5L were incubated with Ni-NTA (Qiagen), Glutathione-Sepharose 4B (GE Life Sciences) or Amylose resin (New England Biolabs) respectively, for 1 h at 4°C, washed and eluted using 1M imidazole, 10 mM reduced glutathione or 50 mM maltose, respectively. .. For gel filtration, crosslinking and HDX, the tags of CWC15 and CDC5L were cleaved off by HRV 3C and Tobacco Etch Virus (TEV) proteases respectively, followed by a second purification step on a 6 ml Resource Q ion exchange column in the case of CTNNBL1 and CWC15, or a Resource S ion exchange column in the case of CDC5L using an AKTA Prime Plus FPLC system (GE Lifesciences). ..



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